Cre drivers

Welcome to the CRE-driver network established by the NIH Neuroscience Blueprint. The goal of the project is to provide the Neuroscience Community with mouse strains that are suitable for the tissue and cell-type-specific perturbation of gene function in the nervous system. The use of experimental animals is widely recognized as a critical.  · Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain Allison S. Mogul, 1, * Colleen K. Hadley, 1, * Haley S. Province, 1, * Jordan Pauli, 2 Oksana Gavrilova, 3 Cuiying Xiao, 1 Richard D. Palmiter, 2 Ramón A. Piñol, 1 and Marc L. Reitman 1Author: Allison S. Mogul, Colleen K. Hadley, Haley S. Province, Jordan Pauli, Oksana Gavrilova, Cuiying Xiao. The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines designed for Cited by:

Large numbers of Cre driver lines with expression in the central nervous system have been generated by projects such as GENSAT (Gong et al., ), the NIH Neuroscience Blueprint Cre Driver Network (Taniguchi et al., ), at the Allen Institute, and by many individual laboratories. Many of these lines have been made publicly available to the broader neuroscience community through repositories such as Jackson Laboratory and MMRRC. These Cre “driver” lines can be used to generate conditional mutations that are activated in distinct cell types, tissues or time points, or inducible mutations that are activated through administration of a drug. More than Cre driver lines have now been created and characterized, targeting selected neuronal populations in the brain. First, Cre-driver strain is generated in which Cre recombinase is expressed by a promoter that specifically targets the cell or tissue of interest. Second, loxP flanked (floxed) DNA containing mouse strain is needed to be generated. Conditional knockout mice are then generated by breeding the Cre-driver strain with a floxed mouse strain (Figure 1B). The specificity and timing of recombination are controlled by used promoter and/or enhancer.

However, the research community requires more cre recombinase expressing transgenic mouse strains (cre-drivers) that restrict expression to specific cell. 22 មករា More specifically, we generated Cre driver rat models that allow controlled gene expression or knockout (conditional models) both temporally. An Australian Centre for Research Excellence (CRE) in “Driving Global Investment in Adolescent Health” has been funded by Australia's National Health and.